Avian influenza viral nucleocapsid and hemagglutinin proteins induce chicken CD8+ memory T lymphocytes.
Virology
; 399(2): 231-8, 2010 Apr 10.
Article
en En
| MEDLINE
| ID: mdl-20116819
ABSTRACT
The avian influenza viruses (AIVs) can be highly contagious to poultry and a zoonotic threat to humans. Since the memory CD8(+) T lymphocyte responses in chickens to AIV proteins have not been defined, these responses to H5N9 AIV hemagglutinin (HA) and nucleocapsid (NP) proteins were evaluated by ex vivo stimulation with virus infected non-professional antigen presenting cells. Secretion of IFNgamma by activated T lymphocytes was evaluated through macrophage induction of nitric oxide. AIV specific, MHC-I restricted memory CD8(+) T lymphocyte responses to NP and HA were observed 3 to 9 weeks post-inoculation (p.i.). The responses specific to NP were greater than those to HA with maximum responses being observed at 5 weeks p.i. followed by a decline to weakly detectable levels by 9 weeks p.i. The cross-reaction of T lymphocytes to a heterologous H7N2 AIV strain demonstrated their ability to respond to a broader range of AIV.
Texto completo:
1
Colección:
01-internacional
Asunto principal:
Linfocitos T CD8-positivos
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Glicoproteínas Hemaglutininas del Virus de la Influenza
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Proteínas de la Nucleocápside
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Gripe Aviar
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Memoria Inmunológica
Límite:
Animals
Idioma:
En
Revista:
Virology
Año:
2010
Tipo del documento:
Article
País de afiliación:
Estados Unidos