Experimental procedure for the characterization of radiation damage in macromolecular crystals.
J Synchrotron Radiat
; 18(Pt 3): 381-6, 2011 May.
Article
en En
| MEDLINE
| ID: mdl-21525646
ABSTRACT
A reliable and reproducible method to automatically characterize the radiation sensitivity of macromolecular crystals at the ESRF beamlines has been developed. This new approach uses the slope of the linear dependence of the overall isotropic B-factor with absorbed dose as the damage metric. The method has been implemented through an automated procedure using the EDNA on-line data analysis framework and the MxCuBE data collection control interface. The outcome of the procedure can be directly used to design an optimal data collection strategy. The results of tests carried out on a number of model and real-life crystal systems are presented.
Texto completo:
1
Colección:
01-internacional
Tipo de estudio:
Prognostic_studies
Idioma:
En
Revista:
J Synchrotron Radiat
Asunto de la revista:
RADIOLOGIA
Año:
2011
Tipo del documento:
Article
País de afiliación:
Francia