Your browser doesn't support javascript.
loading
Functional and transcriptomic recovery of infarcted mouse myocardium treated with bone marrow mononuclear cells.
Stem Cell Rev Rep ; 8(1): 251-61, 2012 Mar.
Article en En | MEDLINE | ID: mdl-21671060
ABSTRACT
Although bone marrow-derived mononuclear cells (BMNC) have been extensively used in cell therapy for cardiac diseases, little mechanistic information is available to support reports of their efficacy. To address this shortcoming, we compared structural and functional recovery and associated global gene expression profiles in post-ischaemic myocardium treated with BMNC transplantation. BMNC suspensions were injected into cardiac scar tissue 10 days after experimental myocardial infarction. Six weeks later, mice undergoing BMNC therapy were found to have normalized antibody repertoire and improved cardiac performance measured by ECG, treadmill exercise time and echocardiography. After functional testing, gene expression profiles in cardiac tissue were evaluated using high-density oligonucleotide arrays. Expression of more than 18% of the 11981 quantified unigenes was significantly altered in the infarcted hearts. BMNC therapy restored expression of 2099 (96.2%) of the genes that were altered by infarction but led to altered expression of 286 other genes, considered to be a side effect of the treatment. Transcriptional therapeutic efficacy, a metric calculated using a formula that incorporates both recovery and side effect of treatment, was 73%. In conclusion, our results confirm a beneficial role for bone marrow-derived cell therapy and provide new information on molecular mechanisms operating after BMNC transplantation on post ischemic heart failure in mice.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Asunto principal: Trasplante de Médula Ósea / Transcriptoma / Corazón / Infarto del Miocardio / Miocardio Límite: Animals Idioma: En Revista: Stem Cell Rev Rep Año: 2012 Tipo del documento: Article País de afiliación: Brasil

Texto completo: 1 Colección: 01-internacional Asunto principal: Trasplante de Médula Ósea / Transcriptoma / Corazón / Infarto del Miocardio / Miocardio Límite: Animals Idioma: En Revista: Stem Cell Rev Rep Año: 2012 Tipo del documento: Article País de afiliación: Brasil