Human peripheral blood CD8+ CD28- T cells of renal allograft recipients do not express FOXP3 protein.
Transplant Proc
; 43(8): 2917-21, 2011 Oct.
Article
en En
| MEDLINE
| ID: mdl-21996189
ABSTRACT
INTRODUCTION:
In recent studies, the FOXP3 molecule has been suggested to be a marker of a suppressor subset of human CD8+ CD28- T cells based on correlations between the level of its mRNA and allograft function. Because this transcriptional factor produces a protein, we suggest that these correlations should focus on the FOXP3 protein. The aim of our study was to evaluate whether FOXP3 protein was present in cells of the CD8+ CD28- population in the peripheral blood of renal allograft recipients and whether the level of CD8+ CD28- FOXP3+ cells correlated with allograft function.METHODS:
The study was performed on 30 renal allograft recipients with uneventful stable courses (n=18) or biopsy-proven chronic rejection (n=12). The immunosuppression was based on cyclosporine (n=12) or rapamycin (n=9). Peripheral blood mononuclear cells isolated from recipient blood samples were labeled with anti-CD8 and anti-CD28 MAbs conjugated with fluorochromes. After incubation, washing, and labeling using a PE anti-human FOXP3 Kit, we determined the percentage of cells by flow cytometry.RESULTS:
FOXP3 protein expression was not observed either in the CD8+ CD28- population, or the whole populations of CD8+ or CD28- cells among patient groups.CONCLUSIONS:
The expression of FOXP3 protein in CD8+ CD28- cells seems to be of a questionable value as a diagnostic tool for allograft function, it is probably not a marker for the CD8+ CD28- T cell subset.
Texto completo:
1
Colección:
01-internacional
Asunto principal:
Subgrupos de Linfocitos T
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Trasplante de Riñón
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Linfocitos T CD8-positivos
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Factores de Transcripción Forkhead
Tipo de estudio:
Observational_studies
Límite:
Adult
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Aged
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Female
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Humans
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Male
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Middle aged
Idioma:
En
Revista:
Transplant Proc
Año:
2011
Tipo del documento:
Article
País de afiliación:
Polonia