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Multi-target siRNA based on DNMT3A/B homologous conserved region influences cell cycle and apoptosis of human prostate cancer cell line TSU-PR1.
Du, Yue-Feng; Liang, Liang; Shi, Ying; Long, Qing-Zhi; Zeng, Jin; Wang, Xin-Yang; He, Da-Lin.
Afiliación
  • Du YF; Department of Urology, First Affiliated Hospital of Medical School, Xi'an Jiaotong University, Xi'an, Shanxi, P.R. China.
Genet Mol Biol ; 35(1): 164-71, 2012 Jan.
Article en En | MEDLINE | ID: mdl-22481891
ABSTRACT
Abnormal genome hypermethylation participates in the tumorigenesis and development of prostate cancer. Prostate cancer cells highly express DNA methyltransferase 3 (DMNT3) family genes, essential for maintaining genome methylation. In the present study, multi-target siRNA, based on the homologous region of the DNMT3 family, was designed for the in vitro investigation of its effects on the proliferation, migration, and invasion of TSU-PR1 prostate cancer cells. The consequential cell-cycle derangement, through DNMT3A/B or only DNMT3B silencing, was partially efficient, without affecting apoptosis. DNMT3A silencing had absolutely no effect on changing TSU-PR1 cell biological behavior. Hence, DNMT3B alone apparently plays a key role in maintaining the unfavorable behavior of prostate-cancer cells, thereby implying its potential significance as a promising therapeutic target, with DNMT3A simply in the role of helper.
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Texto completo: 1 Colección: 01-internacional Idioma: En Revista: Genet Mol Biol Año: 2012 Tipo del documento: Article

Texto completo: 1 Colección: 01-internacional Idioma: En Revista: Genet Mol Biol Año: 2012 Tipo del documento: Article