TGF-ß enhanced IL-21-induced differentiation of human IL-21-producing CD4+ T cells via Smad3.
PLoS One
; 8(5): e64612, 2013.
Article
en En
| MEDLINE
| ID: mdl-23741351
ABSTRACT
IL-21 has pleiotropic effects on innate and adaptive immune response, and plays an important role in the development of autoimmune disease and antitumor activity. It has been reported that IL-21 is produced by CD4(+) T cells and NKT cells. However, the differentiation of IL-21-producing CD4(+) T cells in humans remains largely unclear. In the present study, we showed that cytokines of IL-1ß, IL-6 or IL-21 induced differentiation of human IL-21-producing CD4(+) T cells, and TGF-ß enhanced the effect of inflammatory cytokines on the development of IL-21-producing CD4(+) T cells. Furthermore, we found that the majority of IL-21-producing cells were distinct from Th17 cells and Th1 cells since they did not co-express IL-17 and IFN-γ. TGF-ß significantly inhibited the production of IFN-γ and enhanced the effect of IL-21 on the development of IL-21-producing CD4(+) T cells. In addition, we found that IL-21 inhibited the differentiation of CD4(+) Foxp3(+) T cells induced by TGF-ß. Further study indicated that IL-21 induced phosphorylation of transcriptional factors of STAT1, STAT3 and STAT5, and TGF-ß induced phosphorylation of Smad3 in CD4(+) T cells. Taken together, our data indicated that TGF-ß enhanced IL-21-induced differentiation of IL-21-producing CD4(+) T cells, and the majority of IL-21-producing cells were different from Th17 and Th1 cells. Our results provide a new sight regarding the differentiation of human CD4(+) T cells.
Texto completo:
1
Colección:
01-internacional
Asunto principal:
Linfocitos T CD4-Positivos
/
Factor de Crecimiento Transformador beta
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Interleucinas
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Proteína smad3
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Sangre Fetal
Límite:
Humans
Idioma:
En
Revista:
PLoS One
Asunto de la revista:
CIENCIA
/
MEDICINA
Año:
2013
Tipo del documento:
Article
País de afiliación:
China