Blockade of OX40/OX40 ligand to decrease cytokine messenger RNA expression in acute renal allograft rejection in vitro.
Transplant Proc
; 45(6): 2565-8, 2013.
Article
en En
| MEDLINE
| ID: mdl-23953582
ABSTRACT
AIM:
The aim of this study was to investigate cytokine messenger RNA (mRNA) expression by peripheral blood mononuclear cells (PBMCs) from renal recipients experiencing acute rejection by blocking OX40-OX40L interactions with recombinant human OX40-Fc fusion protein (rhOX40Fc) in vitro.METHODS:
PBMCs were isolated from 20 recipients experiencing acute rejection episodes (rejection group) and 20 recipients with stable graft function (stable group). Levels of Th1 (interferon [IFN]-γ) and Th2 (interleukin [IL]-4) mRNA expressions by PBMCs were measured using real-time reverse transcriptase-polymerase chain reactions.RESULTS:
IFN-γ mRNA expression levels were significantly higher in the rejection than the stable group (P < .05). Levels of IL-4 mRNA expression were not significantly different. Among the rejection group, rhOX40Fc reduced significantly the expression of IFN-γ and IL-4 mRNA by anti-CD3-monoclonal antibody stimulated PBMCs (P < .05, and P < .01, respectively).CONCLUSIONS:
Blocking of the interaction between OX40 and OX40L in vitro inhibited production of Thl and Th2 type cytokines.
Texto completo:
1
Colección:
01-internacional
Asunto principal:
Fragmentos Fc de Inmunoglobulinas
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ARN Mensajero
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Antígenos de Diferenciación
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Citocinas
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Trasplante de Riñón
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Ligando OX40
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Receptores OX40
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Rechazo de Injerto
Tipo de estudio:
Observational_studies
Límite:
Adult
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Female
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Humans
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Male
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Middle aged
Idioma:
En
Revista:
Transplant Proc
Año:
2013
Tipo del documento:
Article
País de afiliación:
China