SPRi-based strategy to identify specific biomarkers in systemic lupus erythematosus, rheumatoid arthritis and autoimmune hepatitis.
PLoS One
; 8(12): e84600, 2013.
Article
en En
| MEDLINE
| ID: mdl-24376828
ABSTRACT
BACKGROUND:
Heterogeneous nuclear ribonucleoprotein (hnRNP) A2/B1 is a target for antinuclear autoantibodies in systemic Lupus erythematosus (SLE), rheumatoid arthritis (RA), and autoimmune hepatitis (AIH).AIM:
To monitor molecular interactions between peptides spanning the entire sequence of hnRNP A2/B1 and sera from patients and healthy controls.METHODS:
Sera from 8 patients from each pathology and controls were passed across a surface plasmon resonance Imagery (SPRi) surface containing 39 overlapping peptides of 17 mers covering the human hnRNP B1. Interactions involving the immobilised peptides were followed in real time and dissociation rate constants k(off) for each interaction were calculated.RESULTS:
Several significant interactions were observed i) high stability (lower k(off) values) between P55â70 and the AIH sera compared to controls (p= 0.003); ii) lower stability (higher k(off) values) between P118â133 and P262â277 and SLE sera, P145â160 and RA sera compared to controls (p=0.006, p=0.002, p=0.007). The binding curves and k(off) values observed after the formation of complexes with anti-IgM and anti-IgG antibodies and after nuclease treatment of the serum indicate that i) IgM isotypes are prevalent and ii) nucleic acids participate in the interaction between anti-hnRNAP B1 and P55â70 and also between controls and the peptides studied.CONCLUSIONS:
These results indicate that P55â70 of hnRNP B1 is a potential biomarker for AIH in immunological tests and suggest the role of circulating nucleic acids, (eg miRNA), present or absent according to the autoimmune disorders and involved in antigen-antibody stability.
Texto completo:
1
Colección:
01-internacional
Asunto principal:
Artritis Reumatoide
/
Biomarcadores
/
Hepatitis Autoinmune
/
Resonancia por Plasmón de Superficie
/
Ribonucleoproteína Heterogénea-Nuclear Grupo A-B
/
Lupus Eritematoso Sistémico
Tipo de estudio:
Prognostic_studies
Límite:
Humans
Idioma:
En
Revista:
PLoS One
Asunto de la revista:
CIENCIA
/
MEDICINA
Año:
2013
Tipo del documento:
Article
País de afiliación:
Francia