Reversible immortalization of human hepatocytes mediated by retroviral transfer and site-specific recombination.
World J Gastroenterol
; 20(36): 13119-26, 2014 Sep 28.
Article
en En
| MEDLINE
| ID: mdl-25278705
ABSTRACT
AIM:
To establish a method for the reversible immortalization of human hepatocytes, which may offer a good and safe source of hepatocytes for practical applications.METHODS:
We successfully isolated primary human hepatocytes from surgically resected liver tissue taken from a patient with liver hemangiomas. The freshly isolated cells were then immortalized with retroviral vector SSR#69 expressing simian virus 40 large T antigen (SV40T) and hygromycin-resistance genes flanked by paired loxP recombination targets.RESULTS:
The freshly isolated hepatocytes with high viability (85%) were successfully immortalized using retroviral gene transfer of SV40T. SV40T in the immortalized cells was then excised by Cre/loxP site-specific recombination. This cell population exhibited the characteristics of differentiated hepatocytes.CONCLUSION:
We successfully established reversibly immortalized human hepatocytes, which will provide an unlimited supply of cells for practical applications.Palabras clave
Texto completo:
1
Colección:
01-internacional
Asunto principal:
Recombinación Genética
/
Retroviridae
/
Transducción Genética
/
Transformación Celular Viral
/
Hepatocitos
Límite:
Humans
Idioma:
En
Revista:
World J Gastroenterol
Asunto de la revista:
GASTROENTEROLOGIA
Año:
2014
Tipo del documento:
Article
País de afiliación:
China