A novel thiol-reductase activity of Arabidopsis YUC6 confers drought tolerance independently of auxin biosynthesis.
Nat Commun
; 6: 8041, 2015 Aug 28.
Article
en En
| MEDLINE
| ID: mdl-26314500
ABSTRACT
YUCCA (YUC) proteins constitute a family of flavin monooxygenases (FMOs), with an important role in auxin (IAA) biosynthesis. Here we report that Arabidopsis plants overexpressing YUC6 display enhanced IAA-related phenotypes and exhibit improved drought stress tolerance, low rate of water loss and controlled ROS accumulation under drought and oxidative stresses. Co-overexpression of an IAA-conjugating enzyme reduces IAA levels but drought stress tolerance is unaffected, indicating that the stress-related phenotype is not based on IAA overproduction. YUC6 contains a previously unrecognized FAD- and NADPH-dependent thiol-reductase activity (TR) that overlaps with the FMO domain involved in IAA biosynthesis. Mutation of a conserved cysteine residue (Cys-85) preserves FMO but suppresses TR activity and stress tolerance, whereas mutating the FAD- and NADPH-binding sites, that are common to TR and FMO domains, abolishes all outputs. We provide a paradigm for a single protein playing a dual role, regulating plant development and conveying stress defence responses.
Texto completo:
1
Colección:
01-internacional
Asunto principal:
Oxidorreductasas
/
Estrés Fisiológico
/
Compuestos de Sulfhidrilo
/
Adaptación Fisiológica
/
Especies Reactivas de Oxígeno
/
Estrés Oxidativo
/
Proteínas de Arabidopsis
/
Sequías
/
Oxigenasas de Función Mixta
/
Ácidos Indolacéticos
Idioma:
En
Revista:
Nat Commun
Asunto de la revista:
BIOLOGIA
/
CIENCIA
Año:
2015
Tipo del documento:
Article