Circulating MicroRNAs: Methodological Aspects in Detection of These Biomarkers.
Folia Biol (Praha)
; 61(6): 203-18, 2015.
Article
en En
| MEDLINE
| ID: mdl-26789142
ABSTRACT
MicroRNAs (miRNAs) are evolutionarily conserved small non-coding RNAs that regulate expression of protein-coding genes involved in important biological processes and (patho)physiological states. Circulating miRNAs are protected against degradation, indicating their relevant biological functions. Many studies have demonstrated an association of the specific profile of circulating miRNAs with a wide range of cancers as well as non-malignant diseases. These findings demonstrate the implication of circulating miRNAs in the pathogenesis of diseases and their potential as non-invasive disease biomarkers. However, methods for measurement of circulating miRNAs have critical technical hotspots, resulting in a discrepancy of the reported results and difficult definition of consensus disease biomarkers that may be implicated in clinical use. Here, we review functions of circulating miRNAs and their aberrant expression in particular diseases. Further, we discuss methodological aspects of their detection and quantification as well as our experience with the methods.
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Colección:
01-internacional
Asunto principal:
Bioquímica
/
Biomarcadores
/
MicroARNs
Tipo de estudio:
Diagnostic_studies
Límite:
Humans
Idioma:
En
Revista:
Folia Biol (Praha)
Año:
2015
Tipo del documento:
Article
País de afiliación:
República Checa