The knee joint loose body as a source of viable autologous human chondrocytes.
Eur J Histochem
; 60(2): 2645, 2016 Jun 10.
Article
en En
| MEDLINE
| ID: mdl-27349321
ABSTRACT
Loose bodies are fragments of cartilage or bone present in the synovial fluid. In the present study we assessed if loose bodies could be used as a source of autologous human chondrocytes for experimental purposes. Histochemical examination of loose bodies and differential enzymatic digestions were undertaken, the isolated cells were cultured in alginate bead microspheres and immunolocalisations were undertaken for chondrogenic markers such as aggrecan, and type II collagen. Isolated loose body cells had high viability (≥90% viable), expressed chondrogenic markers (aggrecan, type II collagen) but no type I collagen. Loose bodies may be a useful source of autologous chondrocytes of high viability.
Texto completo:
1
Colección:
01-internacional
Asunto principal:
Antígenos de Diferenciación
/
Condrocitos
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Cuerpos Libres Articulares
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Articulación de la Rodilla
Límite:
Female
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Humans
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Male
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Middle aged
Idioma:
En
Revista:
Eur J Histochem
Asunto de la revista:
HISTOCITOQUIMICA
Año:
2016
Tipo del documento:
Article