Gigantol from Dendrobium chrysotoxum Lindl. binds and inhibits aldose reductase gene to exert its anti-cataract activity: An in vitro mechanistic study.
J Ethnopharmacol
; 198: 255-261, 2017 Feb 23.
Article
en En
| MEDLINE
| ID: mdl-28104409
ABSTRACT
ETHNOPHARMACOLOGICAL RELEVANCE Dendrobium. chrysotoxum Lindl is a commonly used species of medicinal Dendrobium which belongs to the family of Orchidaceae, locally known as "Shihu" or "Huangcao". D. chrysotoxum Lindl is widely known for medicinal values in traditional Chinese medicine as it possesses anti-inflammatory, anti-hyperglycemic induction, antitumor and antioxidant properties. STUDY AIM:
To characterize the interaction between gigantol extracted from D. chrysotoxum Lindl and the AR gene, and determine gigantol's efficacy against cataractogenesis. MATERIALS ANDMETHODS:
Human lens epithelial cells (HLECs) were induced by glucose as the model group. Reverse transcription polymerase chain reaction (RT-PCR) was used to assess AR gene expression. Then, the mode of interaction of gigantol with the AR gene was evaluated by UV-visible spectroscopy, atomic force microscope (AFM) and surface-enhanced Raman spectroscopy (SERS). The binding constant was determined by UV-visible.RESULTS:
Gigantol depressed AR gene expression in HLECs. UV-visible spectra preliminarily indicated that interaction between the AR gene and gigantol may follow the groove mode, with a binding constant of 1.85×103L/mol. Atomic force microscope (AFM) data indicated that gigantol possibly bound to insert AR gene base pairs of the double helix. Surface-enhanced Raman spectroscopy (SERS) studies further supported these observations.CONCLUSION:
Gigantol extracted from D. chrysotoxum Lindl not only has inhibitory effects on aldose reductase, but also inhibits AR gene expression. These findings provide a more comprehensive theoretical basis for the use of Dendrobium for the treatment of diabetic cataract.Palabras clave
3- Aminopropyl triethoxysilane (PubChem CID: 13521); Aldose reductase (AR) gene; Atomic force microscopy (AFM); D-Glucose (PubChem CID: 579); ETOAC, (PubChem CID: 8857); ETOH, (PubChem CID: 702); Gigantol; Gigantol (PubChem CID: 10221179); Interaction; MEOH, (PubChem CID: 887); Penicillin (PubChem CID: 5904); Streptomycin (PubChem CID: 19649); Surface-enhanced Raman spectroscopy (SERS); UVvisible spectroscopy
Texto completo:
1
Colección:
01-internacional
Asunto principal:
Bibencilos
/
Catarata
/
Aldehído Reductasa
/
Dendrobium
/
Guayacol
Tipo de estudio:
Etiology_studies
/
Prognostic_studies
Límite:
Humans
Idioma:
En
Revista:
J Ethnopharmacol
Año:
2017
Tipo del documento:
Article
País de afiliación:
China