Seq-Well: portable, low-cost RNA sequencing of single cells at high throughput.
Nat Methods
; 14(4): 395-398, 2017 Apr.
Article
en En
| MEDLINE
| ID: mdl-28192419
ABSTRACT
Single-cell RNA-seq can precisely resolve cellular states, but applying this method to low-input samples is challenging. Here, we present Seq-Well, a portable, low-cost platform for massively parallel single-cell RNA-seq. Barcoded mRNA capture beads and single cells are sealed in an array of subnanoliter wells using a semipermeable membrane, enabling efficient cell lysis and transcript capture. We use Seq-Well to profile thousands of primary human macrophages exposed to Mycobacterium tuberculosis.
Texto completo:
1
Colección:
01-internacional
Asunto principal:
Análisis de Secuencia de ARN
/
Análisis de la Célula Individual
/
Secuenciación de Nucleótidos de Alto Rendimiento
Tipo de estudio:
Health_economic_evaluation
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Nat Methods
Asunto de la revista:
TECNICAS E PROCEDIMENTOS DE LABORATORIO
Año:
2017
Tipo del documento:
Article
País de afiliación:
Estados Unidos