Plant homeodomain finger protein 2 as a novel IKAROS target in acute lymphoblastic leukemia.
Epigenomics
; 10(1): 59-69, 2018 01.
Article
en En
| MEDLINE
| ID: mdl-28994305
ABSTRACT
AIM:
Clinical significance of plant homeodomain finger 2 (PHF2) expressions is explored in acute lymphoblastic leukemia (ALL) patients.METHODS:
mRNA level was examined by qPCR. The retroviral gene expression, shRNA knockdown and chromatin-immunoprecipitation are used to observe IKAROS regulation on PHF2 transcription.RESULTS:
PHF2 expression is significantly reduced in subsets of ALL patients, and PHF2 low expression correlates with leukemia cell proliferation and an elevation of several poor prognostic markers in B-cell ALL. IKAROS directly promotes PHF2 expression and patients with IKAROS deletion have significantly lower PHF2 expression. Casein kinase II (CK2) inhibitor significantly promotes PHF2 expression in an IKAROS-dependent manner, and casein kinase II inhibitor treatment also results in an increase of PHF2 expression and enrichment of IKAROS and H3K4me3 at PHF2 promoter in primary cells.CONCLUSION:
Our results demonstrate that the IKAROS promotes PHF2 expression, and suggest that PHF2 low expression works with the IKAROS gene deletion to drive oncogenesis of ALL.Palabras clave
Texto completo:
1
Colección:
01-internacional
Asunto principal:
Proteínas de Homeodominio
/
Factor de Transcripción Ikaros
/
Leucemia-Linfoma Linfoblástico de Células Precursoras
Tipo de estudio:
Prognostic_studies
Límite:
Adolescent
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Adult
/
Aged
/
Child
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Female
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Humans
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Male
/
Middle aged
Idioma:
En
Revista:
Epigenomics
Año:
2018
Tipo del documento:
Article
País de afiliación:
China