Genetic regulation of mitotic competence in G0 quiescent cells.
Sci Adv
; 4(8): eaat5685, 2018 08.
Article
en En
| MEDLINE
| ID: mdl-30116786
ABSTRACT
Quiescent (G0 phase) cells must maintain mitotic competence (MC) to restart the cell cycle. This is essential for reproduction in unicellular organisms and also for development and cell replacement in higher organisms. Recently, suppression of MC has gained attention as a possible therapeutic strategy for cancer. Using a Schizosaccharomyces pombe deletion-mutant library, we identified 85 genes required to maintain MC during the G0 phase induced by nitrogen deprivation. G0 cells must recycle proteins and RNA, governed by anabolism, catabolism, transport, and availability of small molecules such as antioxidants. Protein phosphatases are also essential to maintain MC. In particular, Nem1-Spo7 protects the nucleus from autophagy by regulating Ned1, a lipin. These genes, designated GZE (G-Zero Essential) genes, reveal the landscape of genetic regulation of MC.
Texto completo:
1
Colección:
01-internacional
Asunto principal:
Schizosaccharomyces
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Autofagia
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Regulación Fúngica de la Expresión Génica
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Núcleo Celular
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Fase de Descanso del Ciclo Celular
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Proteínas de Schizosaccharomyces pombe
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Mitosis
Idioma:
En
Revista:
Sci Adv
Año:
2018
Tipo del documento:
Article
País de afiliación:
Japón