Tuning the selectivity of N-alkylated styrylquinolinium dyes for sensing of G-quadruplex DNA.
Bioorg Med Chem
; 27(3): 552-559, 2019 02 01.
Article
en En
| MEDLINE
| ID: mdl-30611633
ABSTRACT
Selective and sensitive detection of G-quadruplex DNA structures is an important issue and attracts extensive interest. To this end, numerous small molecular fluorescent probes have been designed. Here, we present a series of N-alkylated styrylquinolinium dyes named Ls-1, Ls-2 and Ls-3 with varying side groups at the chain end. We found that these dyes exhibited different binding behaviors to DNAs, and Ls-2 with a sulfonato group at the chain end displayed sensitivity and selectivity to G-quadruplex DNA structures in vitro. The characteristics of this dye and its interaction with G-quadruplex DNA were comprehensively investigated by means of UV-vis spectrophotometry, fluorescence, circular dichroism and molecular docking. Furthermore, confocal fluorescence images and MTT assays indicated dye Ls-2 could pass through membrane and enter the living HepG2 cells with low cytotoxicity.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Asunto principal:
Compuestos de Quinolinio
/
Estirenos
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ADN
/
Colorantes Fluorescentes
Límite:
Humans
Idioma:
En
Revista:
Bioorg Med Chem
Asunto de la revista:
BIOQUIMICA
/
QUIMICA
Año:
2019
Tipo del documento:
Article