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miR-34a inhibits esophageal squamous cell carcinoma progression via regulation of FOXM1.
Zhou, Haibo; Yang, Li; Xu, Xinhua; Lu, Mingqian; Guo, Rong; Li, Daojun; Huang, Qiao; Liu, Yang; Deng, Glenn; Xu, Yalin.
Afiliación
  • Zhou H; Institute of Oncology, The First College of Clinical Medical Science, Yichang Central People's Hospital Affiliated to China Three Gorges University, Yichang, Hubei 443000, P.R. China.
  • Yang L; Department of Thyroid and Breast, The First College of Clinical Medical Science, Yichang Central People's Hospital Affiliated to China Three Gorges University, Yichang, Hubei 443000, P.R. China.
  • Xu X; Institute of Oncology, The First College of Clinical Medical Science, Yichang Central People's Hospital Affiliated to China Three Gorges University, Yichang, Hubei 443000, P.R. China.
  • Lu M; Institute of Oncology, The First College of Clinical Medical Science, Yichang Central People's Hospital Affiliated to China Three Gorges University, Yichang, Hubei 443000, P.R. China.
  • Guo R; Institute of Oncology, The First College of Clinical Medical Science, Yichang Central People's Hospital Affiliated to China Three Gorges University, Yichang, Hubei 443000, P.R. China.
  • Li D; Institute of Oncology, The First College of Clinical Medical Science, Yichang Central People's Hospital Affiliated to China Three Gorges University, Yichang, Hubei 443000, P.R. China.
  • Huang Q; Institute of Oncology, The First College of Clinical Medical Science, Yichang Central People's Hospital Affiliated to China Three Gorges University, Yichang, Hubei 443000, P.R. China.
  • Liu Y; Institute of Oncology, The First College of Clinical Medical Science, Yichang Central People's Hospital Affiliated to China Three Gorges University, Yichang, Hubei 443000, P.R. China.
  • Deng G; School of Medicine, Stanford University, Stanford, CA 94305, USA.
  • Xu Y; Advanced Gene Diagnostics, Inc. Tsinghua Science Park, Yichang, Hubei 443001, P.R. China.
Oncol Lett ; 17(1): 706-712, 2019 Jan.
Article en En | MEDLINE | ID: mdl-30655820
ABSTRACT
Downregulation of microRNA-34a (miR-34a) has frequently been observed in esophageal squamous cell carcinoma (ESCC). However, the underlying role and molecular mechanism of miR-34a in ESCC remains largely unknown. In the current study, it was demonstrated that miR-34a was downregulated and forkhead box M1 (FOXM1), a target gene of miR-34a, was upregulated in ESCC tumor tissues. Overexpression of miR-34a decreased FOXM1 mRNA and protein expression in the ESCC cell lines tested (TE-1 and TE-8). Inhibition of miR-34a increased FOXM1 mRNA and protein levels in human esophageal epithelial cells (HEEC). In addition, miR-34a mimics reduced the relative luciferase activity of ESCC cells transfected with FOXM1 3'UTR-WT, but not FOXM1 3'UTR-Mut. The CCK8 assay and scratch wound healing assay showed that overexpression of miR-34a induced inhibition of cell proliferation and cell migration. Additionally, transfection with miR-34a mimics reduced the expression of key genes involved in cell migration (MMP2 and MMP9) in ESCC cells. Thus, the present data demonstrated that miR-34a suppressed ESCC progression by directly targeting FOXM1.
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Texto completo: 1 Colección: 01-internacional Idioma: En Revista: Oncol Lett Año: 2019 Tipo del documento: Article

Texto completo: 1 Colección: 01-internacional Idioma: En Revista: Oncol Lett Año: 2019 Tipo del documento: Article