A flyover style microfluidic chip for highly purified magnetic cell separation.
Biosens Bioelectron
; 129: 175-181, 2019 Mar 15.
Article
en En
| MEDLINE
| ID: mdl-30710755
ABSTRACT
White blood cells (WBCs) isolated from peripheral blood have been verified as important biomarkers for the diagnosis, treatment and prognosis of cancer. However, it's still under challenge to acquire high-purity WBCs, even by taking advantage of current microfluidic technology. Considering the universality of clinical magnetic activated cell sorting (MACS) method, new developments on microfluidic chip in combination of magnetic cells separation technologies may provide a fascinating approach for high-purity WBCs sorting and widely clinical application. Here, we present a flyover style microfluidic chip which has been elaborately embedded with two-stage magnetic separation in continuous flow for WBCs sorting. Immunomagnetic micro/nano-particles (IMNPs) labeled WBC (WBC@IMNPs) were sequentially separated by a lateral magnetic force and a vertical magnetic force, and the final separation purity of WBCs reached up to 93⯱â¯1.67% at a flow rate of 20⯵Lâ¯min-1. Furthermore, the WBCs viability was up to 97.5⯱â¯1.8%. Consequently, this novel flyover style microfluidic-chip with magnetic separation technology has been successfully demonstrated as cut-in-edge method for high-purity WBCs sorting, and obviously it's easy to extend for other types of cells sorting under great potential application in biomedical fields.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Asunto principal:
Separación Inmunomagnética
/
Dispositivos Laboratorio en un Chip
/
Leucocitos
Límite:
Animals
Idioma:
En
Revista:
Biosens Bioelectron
Asunto de la revista:
BIOTECNOLOGIA
Año:
2019
Tipo del documento:
Article