Testing the Impact of Protease Inhibitors in Antigen Presentation Assays.
Methods Mol Biol
; 1988: 59-69, 2019.
Article
en En
| MEDLINE
| ID: mdl-31147932
ABSTRACT
The major histocompatibility complex (MHC) class I restricted pathway of antigen processing allows the presentation of intracellular antigens to cytotoxic T lymphocytes. The proteasome is the main protease in the cytoplasm and the nucleus, which is responsible for the generation of most peptide ligands of MHC-I molecules. Peptides produced by the proteasome can be further trimmed or destroyed by numerous cytosolic or endoplasmic reticulum (ER) luminal proteases. Small molecule inhibitors are useful tools for probing the role of proteases in MHC class I antigen processing. Here, we describe different methods to test the impact of protease inhibitors in antigen presentation assays.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Asunto principal:
Inhibidores de Proteasas
/
Inmunoensayo
/
Presentación de Antígeno
Límite:
Animals
Idioma:
En
Revista:
Methods Mol Biol
Asunto de la revista:
BIOLOGIA MOLECULAR
Año:
2019
Tipo del documento:
Article
País de afiliación:
Suiza