Your browser doesn't support javascript.
loading
Inhibition of CD44 induces apoptosis, inflammation, and matrix metalloproteinase expression in tendinopathy.
Wu, Po-Ting; Su, Wei-Ren; Li, Chia-Lung; Hsieh, Jeng-Long; Ma, Ching-Hou; Wu, Chao-Liang; Kuo, Li-Chieh; Jou, I-Ming; Chen, Shih-Yao.
Afiliación
  • Wu PT; Department of Orthopaedics, College of Medicine, National Cheng Kung University, Tainan, Taiwan.
  • Su WR; Department of Orthopaedics, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan, Taiwan.
  • Li CL; Department of Biomedical Engineering, College of Engineering, National Cheng Kung University, Tainan, Taiwan.
  • Hsieh JL; Department of Orthopaedics, National Cheng Kung University Hospital Dou-Liou Branch, College of Medicine, National Cheng Kung University, Yunlin, Taiwan.
  • Ma CH; Medical Device Innovation Center, National Cheng Kung University, Tainan, Taiwan.
  • Wu CL; Department of Orthopaedics, College of Medicine, National Cheng Kung University, Tainan, Taiwan.
  • Kuo LC; Department of Orthopaedics, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan, Taiwan.
  • Jou IM; Department of Orthopaedics, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan, Taiwan.
  • Chen SY; Department of Orthopaedics, National Cheng Kung University Hospital Dou-Liou Branch, College of Medicine, National Cheng Kung University, Yunlin, Taiwan.
J Biol Chem ; 294(52): 20177-20184, 2019 12 27.
Article en En | MEDLINE | ID: mdl-31732563
ABSTRACT
Apoptosis has emerged as a primary cause of tendinopathy. CD44 signaling pathways exert anti-apoptotic and -inflammatory effects on tumor cells, chondrocytes, and fibroblast-like synoviocytes. The aim of this study was to examine the association among CD44, apoptosis, and inflammation in tendinopathy. Expression of CD44 and apoptotic cell numbers in tendon tissue from patients with long head of biceps (LHB) tendinopathy were determined according to the histological grades of tendinopathy. Primary tenocytes from Achilles tendon of Sprague-Dawley rats 1 week after collagenase injection were cultured with an antagonizing antibody against CD44. Treatment responses were determined by evaluating cell viability and expression of tendon-related proliferation markers, inflammatory mediators, and apoptosis. The expression of CD44 and apoptosis were positively correlated with the severity of tendinopathy in the human LHB tendinopathy. Furthermore, CD44 expression and apoptotic cells were co-stained in tendinopathic tendon. Blocking the CD44 signaling pathways in rat primary tenocytes by OX-50 induced cell apoptosis and the elevated levels of cleaved caspase-3. Furthermore, they had decreased cell viability and expression of collagen type I, type III, tenomodulin, and phosphorylated AKT. In contrast, there were elevated levels of inflammatory mediators, including interleukin (IL)-1ß, IL-6, tumor necrosis factor-α, cyclooxygenase-2, and phosphorylated NF-κB, as well as matrix metalloproteinase (MMP) family members including MMP-1, -3, -9, and -13 in tenocytes upon OX-50 treatment. This study is the first to demonstrate the association of CD44 and apoptosis in tendinopathy. Our data imply that CD44 may play a role in tendinopathy via regulating apoptosis, inflammation, and extracellular matrix homeostasis.
Asunto(s)
Palabras clave

Texto completo: 1 Colección: 01-internacional Asunto principal: Apoptosis / Mediadores de Inflamación / Receptores de Hialuranos / Metaloproteinasas de la Matriz Límite: Animals / Humans / Male Idioma: En Revista: J Biol Chem Año: 2019 Tipo del documento: Article País de afiliación: Taiwán

Texto completo: 1 Colección: 01-internacional Asunto principal: Apoptosis / Mediadores de Inflamación / Receptores de Hialuranos / Metaloproteinasas de la Matriz Límite: Animals / Humans / Male Idioma: En Revista: J Biol Chem Año: 2019 Tipo del documento: Article País de afiliación: Taiwán