A Pre-clinical Rat Model for the Study of Ischemia-reperfusion Injury in Reconstructive Microsurgery.
J Vis Exp
; (153)2019 11 08.
Article
en En
| MEDLINE
| ID: mdl-31762459
ABSTRACT
Ischemia-reperfusion injury is the main cause of flap failure in reconstructive microsurgery. The rat is the preferred preclinical animal model in many areas of biomedical research due to its cost-effectiveness and its translation to humans. This protocol describes a method to create a preclinical free skin flap model in rats with ischemia-reperfusion injury. The described 3 cm x 6 cm rat free skin flap model is easily obtained after the placement of several vascular ligatures and the section of the vascular pedicle. Then, 8 h after the ischemic insult and completion of the microsurgical anastomosis, the free skin flap develops the tissue damage. These ischemia-reperfusion injury-related damages can be studied in this model, making it a suitable model for evaluating therapeutic agents to address this pathophysiological process. Furthermore, two main monitoring techniques are described in the protocol for the assessment of this animal model transit-time ultrasound technology and laser speckle contrast analysis.
Texto completo:
1
Colección:
01-internacional
Asunto principal:
Daño por Reperfusión
/
Procedimientos de Cirugía Plástica
/
Microcirugia
Tipo de estudio:
Guideline
Límite:
Animals
Idioma:
En
Revista:
J Vis Exp
Año:
2019
Tipo del documento:
Article