The multienzymatic protein CAD leading the de novo biosynthesis of pyrimidines localizes exclusively in the cytoplasm and does not translocate to the nucleus.
Nucleosides Nucleotides Nucleic Acids
; 39(10-12): 1320-1334, 2020.
Article
en En
| MEDLINE
| ID: mdl-31997698
ABSTRACT
CAD, the multienzymatic protein that initiates and controls the de novo biosynthesis of pyrimidines, plays a major role in nucleotide homeostasis, cell growth and proliferation. Despite its interest as a potential antitumoral target, there is a lack of understanding on CAD's structure and functioning mechanisms. Although mainly identified as a cytosolic complex, different studies support the translocation of CAD into the nucleus, where it could have a yet undefined function. Here, we track the subcellular localization of CAD by using fluorescent chimeras, cell fractionation and immunoblotting with specific antibodies. Contradicting previous studies, we demonstrate that CAD is exclusively localized at the cytosol and discard a possible translocation to the nucleus.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Asunto principal:
Pirimidinas
/
Aspartato Carbamoiltransferasa
/
Carbamoil-Fosfato Sintasa (Glutamina-Hidrolizante)
/
Núcleo Celular
/
Citoplasma
/
Dihidroorotasa
Límite:
Humans
Idioma:
En
Revista:
Nucleosides Nucleotides Nucleic Acids
Asunto de la revista:
BIOQUIMICA
Año:
2020
Tipo del documento:
Article
País de afiliación:
España