Downregulation of lncRNA ZFAS1 and upregulation of microRNA-129 repress endocrine disturbance, increase proliferation and inhibit apoptosis of ovarian granulosa cells in polycystic ovarian syndrome by downregulating HMGB1.
Genomics
; 112(5): 3597-3608, 2020 09.
Article
en En
| MEDLINE
| ID: mdl-32320818
ABSTRACT
OBJECTIVE:
The objective was to find the role of long-non-coding RNA zinc finger antisense 1 (lncRNA ZFAS1)/microRNA (miR)-129/high-mobility group box protein 1 (HMGB1) axis in polycystic ovary syndrome (PCOS).METHODS:
Ovarian granulosa cells from non-PCOS patients and PCOS patients were collected, and HMGB1, miR-129 and lncRNA ZFAS1 expression were detected. Ovarian granulosa cells were transfected with si-ZFAS1 or miR-129 mimics to verify their roles in P4 and E2 secretion, and the biological functions of ovarian granulosa cells.RESULTS:
LncRNA ZFAS1 and HMGB1 were elevated, while miR-129 was down-regulated in ovarian granulosa cells of PCOS patients. Down-regulated lncRNA ZFAS1 or overexpressed miR-129 could decrease HMGB1 expression, increase P4 and E2 secretion, promote proliferation activity while inhibit apoptosis of ovarian granulosa cells in PCOS.CONCLUSION:
LncRNA ZFAS1 could bind to miR-129 to promote HMGB1 expression, thereby affecting the endocrine disturbance, proliferation and apoptosis of ovarian granulosa cells in PCOS.Palabras clave
Texto completo:
1
Colección:
01-internacional
Asunto principal:
Síndrome del Ovario Poliquístico
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Regulación hacia Abajo
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Regulación hacia Arriba
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Apoptosis
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Proteína HMGB1
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MicroARNs
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Proliferación Celular
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ARN Largo no Codificante
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Células de la Granulosa
Límite:
Female
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Humans
Idioma:
En
Revista:
Genomics
Asunto de la revista:
GENETICA
Año:
2020
Tipo del documento:
Article