Dinucleoside Polyphosphates as RNA Building Blocks with Pairing Ability in Transcription Initiation.
ACS Chem Biol
; 15(7): 1765-1772, 2020 07 17.
Article
en En
| MEDLINE
| ID: mdl-32530599
ABSTRACT
Dinucleoside polyphosphates (NpnNs) were discovered 50 years ago in all cells. They are often called alarmones, even though the molecular target of the alarm has not yet been identified. Recently, we showed that they serve as noncanonical initiating nucleotides (NCINs) and fulfill the role of 5' RNA caps in Escherichia coli. Here, we present molecular insight into their ability to be used as NCINs by T7 RNA polymerase in the initiation phase of transcription. In general, we observed NpnNs to be equally good substrates as canonical nucleotides for T7 RNA polymerase. Surprisingly, the incorporation of ApnGs boosts the production of RNA 10-fold. This behavior is due to the pairing ability of both purine moieties with the -1 and +1 positions of the antisense DNA strand. Molecular dynamic simulations revealed noncanonical pairing of adenosine with the thymine of the DNA.
Texto completo:
1
Colección:
01-internacional
Asunto principal:
ARN
/
Fosfatos de Dinucleósidos
/
Iniciación de la Transcripción Genética
Idioma:
En
Revista:
ACS Chem Biol
Año:
2020
Tipo del documento:
Article
País de afiliación:
República Checa