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Ad26 vaccine protects against SARS-CoV-2 severe clinical disease in hamsters.
Tostanoski, Lisa H; Wegmann, Frank; Martinot, Amanda J; Loos, Carolin; McMahan, Katherine; Mercado, Noe B; Yu, Jingyou; Chan, Chi N; Bondoc, Stephen; Starke, Carly E; Nekorchuk, Michael; Busman-Sahay, Kathleen; Piedra-Mora, Cesar; Wrijil, Linda M; Ducat, Sarah; Custers, Jerome; Atyeo, Caroline; Fischinger, Stephanie; Burke, John S; Feldman, Jared; Hauser, Blake M; Caradonna, Timothy M; Bondzie, Esther A; Dagotto, Gabriel; Gebre, Makda S; Jacob-Dolan, Catherine; Lin, Zijin; Mahrokhian, Shant H; Nampanya, Felix; Nityanandam, Ramya; Pessaint, Laurent; Porto, Maciel; Ali, Vaneesha; Benetiene, Dalia; Tevi, Komlan; Andersen, Hanne; Lewis, Mark G; Schmidt, Aaron G; Lauffenburger, Douglas A; Alter, Galit; Estes, Jacob D; Schuitemaker, Hanneke; Zahn, Roland; Barouch, Dan H.
Afiliación
  • Tostanoski LH; Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, USA.
  • Wegmann F; Janssen Vaccines & Prevention BV, Leiden, Netherlands.
  • Martinot AJ; Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, USA.
  • Loos C; Tufts University Cummings School of Veterinary Medicine, North Grafton, MA, USA.
  • McMahan K; Ragon Institute of MGH, MIT, and Harvard, Cambridge, MA, USA.
  • Mercado NB; Massachusetts Institute of Technology, Cambridge, MA, USA.
  • Yu J; Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, USA.
  • Chan CN; Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, USA.
  • Bondoc S; Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, USA.
  • Starke CE; Oregon Health & Sciences University, Beaverton, OR, USA.
  • Nekorchuk M; Oregon Health & Sciences University, Beaverton, OR, USA.
  • Busman-Sahay K; Oregon Health & Sciences University, Beaverton, OR, USA.
  • Piedra-Mora C; Oregon Health & Sciences University, Beaverton, OR, USA.
  • Wrijil LM; Oregon Health & Sciences University, Beaverton, OR, USA.
  • Ducat S; Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, USA.
  • Custers J; Tufts University Cummings School of Veterinary Medicine, North Grafton, MA, USA.
  • Atyeo C; Tufts University Cummings School of Veterinary Medicine, North Grafton, MA, USA.
  • Fischinger S; Tufts University Cummings School of Veterinary Medicine, North Grafton, MA, USA.
  • Burke JS; Janssen Vaccines & Prevention BV, Leiden, Netherlands.
  • Feldman J; Ragon Institute of MGH, MIT, and Harvard, Cambridge, MA, USA.
  • Hauser BM; Harvard Medical School, Boston, MA, USA.
  • Caradonna TM; Ragon Institute of MGH, MIT, and Harvard, Cambridge, MA, USA.
  • Bondzie EA; Harvard Medical School, Boston, MA, USA.
  • Dagotto G; Ragon Institute of MGH, MIT, and Harvard, Cambridge, MA, USA.
  • Gebre MS; Ragon Institute of MGH, MIT, and Harvard, Cambridge, MA, USA.
  • Jacob-Dolan C; Harvard Medical School, Boston, MA, USA.
  • Lin Z; Ragon Institute of MGH, MIT, and Harvard, Cambridge, MA, USA.
  • Mahrokhian SH; Harvard Medical School, Boston, MA, USA.
  • Nampanya F; Ragon Institute of MGH, MIT, and Harvard, Cambridge, MA, USA.
  • Nityanandam R; Harvard Medical School, Boston, MA, USA.
  • Pessaint L; Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, USA.
  • Porto M; Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, USA.
  • Ali V; Harvard Medical School, Boston, MA, USA.
  • Benetiene D; Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, USA.
  • Tevi K; Harvard Medical School, Boston, MA, USA.
  • Andersen H; Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, USA.
  • Lewis MG; Harvard Medical School, Boston, MA, USA.
  • Schmidt AG; Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, USA.
  • Lauffenburger DA; Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, USA.
  • Alter G; Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, USA.
  • Estes JD; Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, USA.
  • Schuitemaker H; Bioqual, Rockville, MD, USA.
  • Zahn R; Bioqual, Rockville, MD, USA.
  • Barouch DH; Bioqual, Rockville, MD, USA.
Nat Med ; 26(11): 1694-1700, 2020 11.
Article en En | MEDLINE | ID: mdl-32884153
ABSTRACT
Coronavirus disease 2019 (COVID-19) in humans is often a clinically mild illness, but some individuals develop severe pneumonia, respiratory failure and death1-4. Studies of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection in hamsters5-7 and nonhuman primates8-10 have generally reported mild clinical disease, and preclinical SARS-CoV-2 vaccine studies have demonstrated reduction of viral replication in the upper and lower respiratory tracts in nonhuman primates11-13. Here we show that high-dose intranasal SARS-CoV-2 infection in hamsters results in severe clinical disease, including high levels of virus replication in tissues, extensive pneumonia, weight loss and mortality in a subset of animals. A single immunization with an adenovirus serotype 26 vector-based vaccine expressing a stabilized SARS-CoV-2 spike protein elicited binding and neutralizing antibody responses and protected against SARS-CoV-2-induced weight loss, pneumonia and mortality. These data demonstrate vaccine protection against SARS-CoV-2 clinical disease. This model should prove useful for preclinical studies of SARS-CoV-2 vaccines, therapeutics and pathogenesis.
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Texto completo: 1 Colección: 01-internacional Asunto principal: Adenoviridae / Glicoproteína de la Espiga del Coronavirus / Vacunas contra la COVID-19 / SARS-CoV-2 / COVID-19 Límite: Animals / Female / Humans / Male Idioma: En Revista: Nat Med Asunto de la revista: BIOLOGIA MOLECULAR / MEDICINA Año: 2020 Tipo del documento: Article País de afiliación: Estados Unidos
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Texto completo: 1 Colección: 01-internacional Asunto principal: Adenoviridae / Glicoproteína de la Espiga del Coronavirus / Vacunas contra la COVID-19 / SARS-CoV-2 / COVID-19 Límite: Animals / Female / Humans / Male Idioma: En Revista: Nat Med Asunto de la revista: BIOLOGIA MOLECULAR / MEDICINA Año: 2020 Tipo del documento: Article País de afiliación: Estados Unidos