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Toxicity assessment of saline extract and lectin-rich fraction from Microgramma vacciniifolia rhizome.
da Silva, Gabriela Cavalcante; de Oliveira, Alisson Macário; Machado, Janaina Carla Barbosa; Ferreira, Magda Rhayanny Assunção; de Medeiros, Paloma Lys; Soares, Luiz Alberto Lira; de Souza, Ivone Antônia; Paiva, Patrícia Maria Guedes; Napoleão, Thiago Henrique.
Afiliación
  • da Silva GC; Departamento de Bioquímica, Centro de Biociências, Universidade Federal de Pernambuco, Recife, Pernambuco, Brazil.
  • de Oliveira AM; Departamento de Bioquímica, Centro de Biociências, Universidade Federal de Pernambuco, Recife, Pernambuco, Brazil; Departamento de Farmácia, Centro de Ciências da Saúde, Universidade Federal de Pernambuco, Recife, Pernambuco, Brazil.
  • Machado JCB; Departamento de Farmácia, Centro de Ciências da Saúde, Universidade Federal de Pernambuco, Recife, Pernambuco, Brazil.
  • Ferreira MRA; Departamento de Farmácia, Centro de Ciências da Saúde, Universidade Federal de Pernambuco, Recife, Pernambuco, Brazil.
  • de Medeiros PL; Departamento de Histologia e Embriologia, Centro de Biociências, Universidade Federal de Pernambuco, Recife, Pernambuco, Brazil.
  • Soares LAL; Departamento de Farmácia, Centro de Ciências da Saúde, Universidade Federal de Pernambuco, Recife, Pernambuco, Brazil.
  • de Souza IA; Departamento de Antibióticos, Centro de Biociências, Universidade Federal de Pernambuco, Recife, Pernambuco, Brazil.
  • Paiva PMG; Departamento de Bioquímica, Centro de Biociências, Universidade Federal de Pernambuco, Recife, Pernambuco, Brazil.
  • Napoleão TH; Departamento de Bioquímica, Centro de Biociências, Universidade Federal de Pernambuco, Recife, Pernambuco, Brazil. Electronic address: thiago.napoleao@ufpe.br.
Toxicon ; 187: 65-74, 2020 Nov.
Article en En | MEDLINE | ID: mdl-32890585
ABSTRACT
Microgramma vacciniifolia is broadly used in folk medicine but safety information is unavailable. Therefore, we evaluated the toxicity of a saline extract and a lectin-rich fraction of M. vacciniifolia rhizome. The extract showed hemolytic activity on mice erythrocytes at 1000 µg/mL, whereas the fraction promoted hemolysis (8.57-26.15%) at all tested concentrations (10-1000 µg/mL). Acute toxicity test in mice indicated an LD50 of >5000 mg/kg. Hematological alterations and increased serum alkaline phosphatase level were observed in the treated animals. Transaminases and urea levels increased in the groups treated with the extract or fraction at 5000 mg/kg. Leukocyte infiltration was observed in the liver of extract-treated animals and in the liver and lungs of mice treated with the fraction. The kidneys of animals treated with the fraction at 5000 mg/kg presented hydropic degeneration. The extract and fraction did not induce oxidative stress in the liver and did not show genotoxicity, as examined by micronucleus and comet assays. In conclusion, the preparations were not lethal to mice but caused some signs of toxicity, mainly the fraction. The results indicated the need to evaluate the toxicity of M. vacciniifolia rhizome in other models and in chronic assays.
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Texto completo: 1 Colección: 01-internacional Asunto principal: Extractos Vegetales / Polypodiaceae / Rizoma Límite: Animals Idioma: En Revista: Toxicon Año: 2020 Tipo del documento: Article País de afiliación: Brasil

Texto completo: 1 Colección: 01-internacional Asunto principal: Extractos Vegetales / Polypodiaceae / Rizoma Límite: Animals Idioma: En Revista: Toxicon Año: 2020 Tipo del documento: Article País de afiliación: Brasil