Pinealectomy increases thermogenesis and decreases lipogenesis.
Mol Med Rep
; 22(5): 4289-4297, 2020 Nov.
Article
en En
| MEDLINE
| ID: mdl-33000192
ABSTRACT
The present study was designed to determine the effects of pineal glandderived melatonin on obesity by employing a rat pinealectomy (Pnx) model. After 10 weeks of a highfat diet, rats received sham or Pnx surgery followed by a normal chow diet for 10 weeks. Reverse transcriptionquantitative PCR, western blotting analysis, immunohistochemistry and ELISA were used to determine the effects of Pnx. Pnx decreased the expression of melatonin receptor (MTNR)1A and MTNR1B, in brown adipose tissues (BAT) and white adipose tissues (WAT). Pnx rats showed increased insulin sensitivity compared with those that received sham surgery. Leptin levels were significantly decreased in the serum of the Pnx group. In addition, Pnx stimulated thermogenic genes in BAT and attenuated lipogenic genes in both WAT and the liver. Histological analyses revealed a marked decrease in the size of lipid droplets and increased expression of uncoupling protein 1 in BAT. In the liver of the Pnx group, the size and number of lipid droplets had also decreased. In conclusion, the results presented in the current study suggested that Pnx increases thermogenesis in BAT and decreases lipogenesis in WAT and the liver.
Texto completo:
1
Colección:
01-internacional
Asunto principal:
Termogénesis
/
Lipogénesis
/
Pinealectomía
/
Obesidad
Tipo de estudio:
Etiology_studies
Límite:
Animals
Idioma:
En
Revista:
Mol Med Rep
Año:
2020
Tipo del documento:
Article