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MiR-1231 enhances docetaxel sensitivity to gallbladder carcinoma cells by downregulating FOXC2.
Gong, Y-Q; Ni, J-L; Fang, Q; Li, T.
Afiliación
  • Gong YQ; Department of Pharmacy, Guangrao People's Hospital, Dongying, China. 11124719@cumt.edu.cn.
Eur Rev Med Pharmacol Sci ; 24(23): 12116-12123, 2020 12.
Article en En | MEDLINE | ID: mdl-33336729
ABSTRACT

OBJECTIVE:

To illustrate the role of microRNA-1231 (miR-1231) in regulating malignant proliferative potential and DTX sensitivity to gallbladder carcinoma (GBC) by regulating FOXC2 level. PATIENTS AND

METHODS:

Expression levels of miR-1231 in GBC tissues and paracancerous ones were detected. The relationship between miR-1231 level and clinical parameters of GBC patients was analyzed. After overexpression of miR-1231, changes in proliferative and apoptotic potentials in GBC-SD and NOZ cells were examined by Cell Counting Kit-8 (CCK-8), colony formation assay and flow cytometry, respectively. Regulatory effects of miR-1231 on its downstream gene FOXC2 were determined by Luciferase assay. Finally, the role of miR-1231 in regulating DTX sensitivity to GBC cells was assessed.

RESULTS:

MiR-1231 was downregulated in GBC tissues compared to paracancerous ones. GBC patients expressing lower level of miR-1231 had worse tumor staging and larger tumor size. Overexpression of miR-1231 attenuated proliferative potential, and induced apoptosis in GBC cells. FOXC2 was upregulated in GBC and negatively linked to miR-1231. Luciferase activity confirmed that FOXC2 was the target gene binding miR-1231. DTX treatment dose-dependently suppressed viability in GBC cells and overexpression of miR-1231 could enhance DTX sensitivity in GBC. Notably, overexpression of FOXC2 abolished regulatory effects of overexpressed miR-1231 on proliferative and apoptotic potentials in GBC cells.

CONCLUSIONS:

MiR-1231 is downregulated in GBC species. Its level is closely linked to tumor staging and tumor size in GBC patients. By downregulating FOXC2, miR-1231 enhances DTX sensitivity to GBC cells and thus alleviates the malignant development of GBC.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Asunto principal: Regulación hacia Abajo / MicroARNs / Factores de Transcripción Forkhead / Neoplasias de la Vesícula Biliar Tipo de estudio: Diagnostic_studies Límite: Female / Humans / Male / Middle aged Idioma: En Revista: Eur Rev Med Pharmacol Sci Asunto de la revista: FARMACOLOGIA / TOXICOLOGIA Año: 2020 Tipo del documento: Article País de afiliación: China

Texto completo: 1 Colección: 01-internacional Asunto principal: Regulación hacia Abajo / MicroARNs / Factores de Transcripción Forkhead / Neoplasias de la Vesícula Biliar Tipo de estudio: Diagnostic_studies Límite: Female / Humans / Male / Middle aged Idioma: En Revista: Eur Rev Med Pharmacol Sci Asunto de la revista: FARMACOLOGIA / TOXICOLOGIA Año: 2020 Tipo del documento: Article País de afiliación: China