Mitochondrial Bioenergetic Assays as a Standard Protocol for Toxicological and Metabolic Assessment.
Methods Mol Biol
; 2240: 231-241, 2021.
Article
en En
| MEDLINE
| ID: mdl-33423237
ABSTRACT
Mitochondria are the center for all metabolic pathways within the eukaryotic cell. Being responsible for the production of over 95% of the cell's requirement of adenosine triphosphate any effect on the function of mitochondria is sure to cause disruption of cellular activity and even viability. As such, it comes as no surprise that many diseases have mitochondrial dysfunction at their core. Understanding mitochondrial function and capacity in the context of a study is key for perceiving and explaining the behavior of said disease or toxic effect. Here, we describe a wide array of simple and yet elegant assays that can be easily implemented to ascertain the function of mitochondria and thus greatly improve the understanding of how a certain disease or compound causes its effects on the cellular function.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Asunto principal:
Bioensayo
/
Mitocondrias Hepáticas
/
Pruebas de Toxicidad
/
Metabolismo Energético
Límite:
Animals
Idioma:
En
Revista:
Methods Mol Biol
Asunto de la revista:
BIOLOGIA MOLECULAR
Año:
2021
Tipo del documento:
Article
País de afiliación:
Portugal