Determination of Trimethylamine N-oxide and Betaine in Serum and Food by Targeted Metabonomics.
Molecules
; 26(5)2021 Mar 02.
Article
en En
| MEDLINE
| ID: mdl-33801417
ABSTRACT
Trimethylamine N-oxide (TMAO), as a gut-derived metabolite, has been found to be associated with enhanced risk for atherosclerosis and cardiovascular disease. We presented a method for targeted profiling of TMAO and betaine in serum and food samples based on a combination of one-step sample pretreatment and proton nuclear magnetic resonance spectroscopy. The key step included a processing of sample preparation using a selective solid-phase extraction column for retention of basic metabolites. Proton signals at δ 3.29 and δ 3.28 were employed to quantify TMAO and betaine, respectively. The developed method was examined with acceptable linear relationship, precision, stability, repeatability, and accuracy. It was successfully applied to detect serum levels of TMAO and betaine in TMAO-fed mice and high-fructose-fed rats and also used to determine the contents of TMAO and betaine in several kinds of food, such as fish, pork, milk, and egg yolk.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Asunto principal:
Óxidos
/
Betaína
/
Metabolómica
/
Análisis de los Alimentos
/
Metilaminas
Límite:
Animals
Idioma:
En
Revista:
Molecules
Asunto de la revista:
BIOLOGIA
Año:
2021
Tipo del documento:
Article
País de afiliación:
China