CRISPR Interference Efficiently Silences Latent and Lytic Viral Genes in Kaposi's Sarcoma-Associated Herpesvirus-Infected Cells.
Viruses
; 13(5)2021 04 28.
Article
en En
| MEDLINE
| ID: mdl-33924938
ABSTRACT
Uncovering viral gene functions requires the modulation of gene expression through overexpression or loss-of-function. CRISPR interference (CRISPRi), a modification of the CRISPR-Cas9 gene editing technology, allows specific and efficient transcriptional silencing without genetic ablation. CRISPRi has been used to silence eukaryotic and prokaryotic genes at the single-gene and genome-wide levels. Here, we report the use of CRISPRi to silence latent and lytic viral genes, with an efficiency of ~80-90%, in epithelial and B-cells carrying multiple copies of the Kaposi's sarcoma-associated herpesvirus (KSHV) genome. Our results validate CRISPRi for the analysis of KSHV viral elements, providing a functional genomics tool for studying virus-host interactions.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Asunto principal:
Activación Viral
/
Latencia del Virus
/
Herpesvirus Humano 8
/
Silenciador del Gen
/
Repeticiones Palindrómicas Cortas Agrupadas y Regularmente Espaciadas
/
Edición Génica
Tipo de estudio:
Risk_factors_studies
Límite:
Humans
Idioma:
En
Revista:
Viruses
Año:
2021
Tipo del documento:
Article
País de afiliación:
Estados Unidos