Development of a high-level light-activated disinfectant for hard surfaces and medical devices.
Int J Antimicrob Agents
; 58(1): 106360, 2021 Jul.
Article
en En
| MEDLINE
| ID: mdl-33992750
ABSTRACT
BACKGROUND:
Bacterial spores are an important consideration in healthcare decontamination, with cross-contamination highlighted as a major route of transmission due to their persistent nature. Their containment is extremely difficult due to the toxicity and cost of first-line sporicides.METHODS:
Susceptibility of Staphylococcus aureus, Bacillus subtilis, Pseudomonas aeruginosa and Escherichia coli to phenothiazinium photosensitizers and cationic surfactants under white- or red-light irradiation was assessed by determination of minimum inhibitory concentrations, minimum bactericidal concentrations and time-kill assays. B. subtilis spore eradication was assessed via time-kill assays, with and without nutrient and non-nutrient germinant supplementation of photosensitizer, surfactant and photosensitizer-surfactant solutions in the presence and absence of light.RESULTS:
Under red-light irradiation, >5-log10 colony-forming units/mL reduction of vegetative bacteria was achieved within 10 min with toluidine blue O (TBO) and methylene blue (MB). Cationic surfactant addition did not significantly enhance spore eradication by photosensitizers (P>0.05). However, addition of a nutrient germinant mixture to TBO achieved a 6-log10 reduction after 20 min of irradiation, while providing 1-2 log10 improvement in spore eradication for MB and pyronin Y.CONCLUSIONS:
Light-activated photosensitizer solutions in the presence of surfactants and germination-promoting agents provide a highly effective method to eradicate dormant and vegetative bacteria. These solutions could provide a useful alternative to traditional chemical agents used for high-level decontamination and infection control within health care.Palabras clave
Texto completo:
1
Colección:
01-internacional
Asunto principal:
Esporas Bacterianas
/
Tensoactivos
/
Bacterias
/
Esterilización
/
Fármacos Fotosensibilizantes
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Desinfectantes
/
Equipos y Suministros
Límite:
Humans
Idioma:
En
Revista:
Int J Antimicrob Agents
Año:
2021
Tipo del documento:
Article
País de afiliación:
Reino Unido