Intracellular IL-33 Attenuates Extracellular IL-33-induced Cholangiocarcinoma Cell Proliferation and Invasion via NF-κB and GSK-3ß Pathways.
Anticancer Res
; 41(10): 4917-4928, 2021 Oct.
Article
en En
| MEDLINE
| ID: mdl-34593439
ABSTRACT
BACKGROUND/AIM:
The functions of interleukin 33 (IL-33) in cholangiocarcinoma (CCA) are unclear. This study aimed to evaluate the roles of IL-33 in CCA progression. MATERIALS ANDMETHODS:
The effect of intracellular IL-33 using shIL-33 knocked down KKU-055 (IL-33KD-KKU-055) compared to parental (Pa) KKU-055 and extracellular IL-33 using recombinant human IL-33 (rhIL-33) treatment on the proliferation and invasion of CCA cells grown in 3D cultures was studied. Relevant markers were determined by western blot or ELISA.RESULTS:
IL-33KD-KKU-055 cells showed increased proliferation and invasion in 3D cultures compared to Pa-KKU-055 cells, with NF-κB and IL-6 up-regulation. Treatment with 2 ng/ml rhIL-33 promoted Pa-KKU-055 cell proliferation by inducing NF-κB and IL-6 expressions. Upon GSK-3ß inactivation and increased nuclear full-length IL-33 (flIL-33), 20 ng/ml rhIL-33 had no effect on proliferation. Both 2 and 20 ng/ml rhIL-33 induced proliferation and invasion of IL-33-negative KKU-213 cells in 3D cultures, as well as NF-κB and IL-6 up-regulation.CONCLUSION:
Intracellular and extracellular IL-33 have distinct roles in the mechanisms of CCA progression.Palabras clave
Texto completo:
1
Colección:
01-internacional
Asunto principal:
Neoplasias de los Conductos Biliares
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Biomarcadores de Tumor
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Regulación Neoplásica de la Expresión Génica
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FN-kappa B
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Colangiocarcinoma
/
Interleucina-33
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Glucógeno Sintasa Quinasa 3 beta
Tipo de estudio:
Prognostic_studies
Límite:
Humans
Idioma:
En
Revista:
Anticancer Res
Año:
2021
Tipo del documento:
Article
País de afiliación:
Tailandia