Synthesis of the l- and d-SH2 domain of the leukaemia oncogene Bcr-Abl.
RSC Chem Biol
; 3(8): 1008-1012, 2022 Aug 03.
Article
en En
| MEDLINE
| ID: mdl-35975004
ABSTRACT
The d- and l-versions of the Bcr-Abl SH2 domain (12.7 kDa) were synthesized. Key optimizations included pseudoproline incorporation, N-terminal hydrophilic tail addition and mild N-acetoxy succinimide acetylation. Their folding and activity are as for the recombinant protein. Our results will enable engineering of mirror-image monobody antagonists of the central oncoprotein Bcr-Abl.
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01-internacional
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En
Revista:
RSC Chem Biol
Año:
2022
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Article