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Elevated and Sustained Intracellular Calcium Signalling Is Necessary for Efficacious Induction of the Human Sperm Acrosome Reaction.
Prajapati, Priyanka; Kane, Shruti; McBrinn, Rachel C; Dean, Morven S; Martins da Silva, Sarah J; Brown, Sean G.
Afiliación
  • Prajapati P; Reproductive Medicine Research Group, School of Medicine, Ninewells Hospital and Medical School, University of Dundee, Dundee DD1 9SY, UK.
  • Kane S; School of Applied Sciences, Abertay University, Dundee DD1 1HG, UK.
  • McBrinn RC; School of Applied Sciences, Abertay University, Dundee DD1 1HG, UK.
  • Dean MS; Reproductive Medicine Research Group, School of Medicine, Ninewells Hospital and Medical School, University of Dundee, Dundee DD1 9SY, UK.
  • Martins da Silva SJ; Assisted Conception Unit, Ninewells Hospital, Dundee DD1 9SY, UK.
  • Brown SG; Reproductive Medicine Research Group, School of Medicine, Ninewells Hospital and Medical School, University of Dundee, Dundee DD1 9SY, UK.
Int J Mol Sci ; 23(19)2022 Sep 24.
Article en En | MEDLINE | ID: mdl-36232560
ABSTRACT
Progesterone and prostaglandin E1 are postulated to trigger the human sperm acrosome reaction (AR). However, their reported efficacy is very variable which likely, in part, reflects the plethora of experimental conditions and methodologies used to detect this physiologically relevant event. The purpose of this study was to develop an assay for the robust induction and objective measurement of the complete AR. Sperm from healthy volunteers or patients undertaking IVF were treated with a variety of ligands (progesterone, prostaglandin E1 or NH4Cl, alone or in combinations). AR, motility and intracellular calcium measurements were measured using flow cytometry, computer-assisted sperm analysis (CASA) and fluorimetry, respectively. The AR was significantly increased by the simultaneous application of progesterone, prostaglandin E1 and NH4Cl, following an elevated and sustained intracellular calcium concentration. However, we observed notable inter- and intra-donor sample heterogeneity of the AR induction. When studying the patient samples, we found no relationship between the IVF fertilization rate and the AR. We conclude that progesterone and prostaglandin E1 alone do not significantly increase the percentage of live acrosome-reacted sperm. This assay has utility for drug discovery and sperm toxicology studies but is not predictive for IVF success.
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Texto completo: 1 Colección: 01-internacional Asunto principal: Calcio / Reacción Acrosómica Límite: Humans / Male Idioma: En Revista: Int J Mol Sci Año: 2022 Tipo del documento: Article País de afiliación: Reino Unido

Texto completo: 1 Colección: 01-internacional Asunto principal: Calcio / Reacción Acrosómica Límite: Humans / Male Idioma: En Revista: Int J Mol Sci Año: 2022 Tipo del documento: Article País de afiliación: Reino Unido