Your browser doesn't support javascript.
loading
Matrin3 regulates mitotic spindle dynamics by controlling alternative splicing of CDC14B.
Muys, Bruna R; Shrestha, Roshan L; Anastasakis, Dimitrios G; Pongor, Lorinc; Li, Xiao Ling; Grammatikakis, Ioannis; Polash, Ahsan; Chari, Raj; Gorospe, Myriam; Harris, Curtis C; Aladjem, Mirit I; Basrai, Munira A; Hafner, Markus; Lal, Ashish.
Afiliación
  • Muys BR; Regulatory RNAs and Cancer Section, Genetics Branch, Center for Cancer Research (CCR), National Cancer Institute (NCI), Bethesda, MD 20892, USA.
  • Shrestha RL; Genetics Branch, CCR, NCI, Bethesda, MD 20892, USA.
  • Anastasakis DG; RNA Molecular Biology Laboratory, National Institute for Arthritis and Musculoskeletal and Skin Disease, Bethesda, MD 20892, USA.
  • Pongor L; Developmental Therapeutics Branch, CCR, NCI, NIH, Bethesda, MD 20892, USA.
  • Li XL; Regulatory RNAs and Cancer Section, Genetics Branch, Center for Cancer Research (CCR), National Cancer Institute (NCI), Bethesda, MD 20892, USA.
  • Grammatikakis I; Regulatory RNAs and Cancer Section, Genetics Branch, Center for Cancer Research (CCR), National Cancer Institute (NCI), Bethesda, MD 20892, USA.
  • Polash A; RNA Molecular Biology Laboratory, National Institute for Arthritis and Musculoskeletal and Skin Disease, Bethesda, MD 20892, USA.
  • Chari R; Genome Modification Core, Frederick National Lab for Cancer Research, Frederick, MD 21701, USA.
  • Gorospe M; Laboratory of Genetics and Genomics, National Institute on Aging, Baltimore, MD 21224, USA.
  • Harris CC; Laboratory of Human Carcinogenesis, CCR, NCI, Bethesda, MD 20892, USA.
  • Aladjem MI; Developmental Therapeutics Branch, CCR, NCI, NIH, Bethesda, MD 20892, USA.
  • Basrai MA; Genetics Branch, CCR, NCI, Bethesda, MD 20892, USA.
  • Hafner M; RNA Molecular Biology Laboratory, National Institute for Arthritis and Musculoskeletal and Skin Disease, Bethesda, MD 20892, USA. Electronic address: markus.hafner@nih.gov.
  • Lal A; Regulatory RNAs and Cancer Section, Genetics Branch, Center for Cancer Research (CCR), National Cancer Institute (NCI), Bethesda, MD 20892, USA. Electronic address: ashish.lal@nih.gov.
Cell Rep ; 42(3): 112260, 2023 03 28.
Article en En | MEDLINE | ID: mdl-36924503
ABSTRACT
Matrin3 is an RNA-binding protein that regulates diverse RNA-related processes, including mRNA splicing. Although Matrin3 has been intensively studied in neurodegenerative diseases, its function in cancer remains unclear. Here, we report Matrin3-mediated regulation of mitotic spindle dynamics in colorectal cancer (CRC) cells. We comprehensively identified RNAs bound and regulated by Matrin3 in CRC cells and focused on CDC14B, one of the top Matrin3 targets. Matrin3 knockdown results in increased inclusion of an exon containing a premature termination codon in the CDC14B transcript and simultaneous down-regulation of the standard CDC14B transcript. Knockdown of CDC14B phenocopies the defects in mitotic spindle dynamics upon Matrin3 knockdown, and the elongated and misoriented mitotic spindle observed upon Matrin3 knockdown are rescued upon overexpression of CDC14B, suggesting that CDC14B is a key downstream effector of Matrin3. Collectively, these data reveal a role for the Matrin3/CDC14B axis in control of mitotic spindle dynamics.
Asunto(s)
Palabras clave
Texto completo
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Asunto principal: Empalme Alternativo / Fosfatasas de Especificidad Dual Idioma: En Revista: Cell Rep Año: 2023 Tipo del documento: Article País de afiliación: Estados Unidos
Texto completo
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Asunto principal: Empalme Alternativo / Fosfatasas de Especificidad Dual Idioma: En Revista: Cell Rep Año: 2023 Tipo del documento: Article País de afiliación: Estados Unidos