Sample Preparation and Differential Gene Expression Analysis of Human Cancer Cell Lines by RNA Sequencing.
Methods Mol Biol
; 2660: 23-41, 2023.
Article
en En
| MEDLINE
| ID: mdl-37191788
ABSTRACT
RNA sequencing (RNA-seq) is a method used for the high-throughput quantification of mRNA in a biological sample. It is widely used to investigate differential gene expression between drug-resistant and sensitive cancers to identify genetic mediators of drug resistance. Here, we describe a comprehensive experimental and bioinformatic pipeline to isolate mRNA from human cell lines, prepare mRNA libraries for next-generation sequencing, and perform post-sequencing bioinformatics analyses.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Asunto principal:
Biología Computacional
/
Neoplasias
Límite:
Humans
Idioma:
En
Revista:
Methods Mol Biol
Asunto de la revista:
BIOLOGIA MOLECULAR
Año:
2023
Tipo del documento:
Article
País de afiliación:
Estados Unidos