Protocol for profiling in vitro intratumor heterogeneity using spatially annotated single-cell sequencing.
STAR Protoc
; 4(3): 102447, 2023 Sep 15.
Article
en En
| MEDLINE
| ID: mdl-37453069
ABSTRACT
Here, we present a protocol for spatially annotated single-cell sequencing, a technique for spatially profiling intratumor heterogeneity with deep single-cell RNA sequencing and single-cell resolution. By combining live-cell imaging and photopatterned illumination, we describe steps to identify regions of interest in an in vitro tumor model, label the selected cells with photoactivatable dyes, and isolate and subject them to scRNAseq. This protocol can be applied to a range of cell lines and could be expanded to tissue sections. For complete details on the use and execution of this protocol, please refer to Smit et al. (2022).1.
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01-internacional
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STAR Protoc
Año:
2023
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Article
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Países Bajos