Impact of Aluminium phthalocyanine nanoconjugate on melanoma stem cells.
Artif Cells Nanomed Biotechnol
; 51(1): 334-345, 2023 Dec.
Article
en En
| MEDLINE
| ID: mdl-37455406
ABSTRACT
Metastatic melanoma cancer stem cells are subpopulations linked to tumour development, immunoevasive behaviour, treatment resistance, and metastasis, all of which contribute to a poor prognosis. Photodynamic treatment (PDT) is an alternate strategy to cancer eradication that involves the generation of reactive oxygen species. As a carrier, nanoparticles enable efficient cellular uptake of photosensitizers, improving organelle accumulation and cancer cell targeted therapy. This study considered at the effect of PDT on CD133+ Melanoma Stem Cells utilising an Aluminium Phthalocyanine Gold Nanoparticle (AlPcS4Cl-AuNP) combination. A ligand exchange approach was used to conjugate AlPcS4Cl-PEG-AuNP-COOH and was characterised using UV-Vis, FTIR, DLS and Zeta Potential. Stem cells isolated from the A375 cell line irradiated with a laser at 673.2 nm with a fluency of 5 J/cm2 were evaluated. Furthermore, it was important to study if apoptosis was one of the mechanisms causing to cell death which was substantiated with Annexin V/PI, caspase 3 and p53 analysis. The nanoparticle conjugate mediated PDT promoted apoptotic cell death, showing increased expression of p53 and caspase-3. The study proposed a strategy aimed at extending the understanding of PDT in enhancing the therapy of melanoma, suggesting a probable improved cell death when AlPcS4Cl was conjugated to AuNPs.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Asunto principal:
Fotoquimioterapia
/
Nanopartículas del Metal
/
Melanoma
Límite:
Humans
Idioma:
En
Revista:
Artif Cells Nanomed Biotechnol
Año:
2023
Tipo del documento:
Article
País de afiliación:
Sudáfrica