Graphene oxide-induced, reactive oxygen species-mediated mitochondrial dysfunctions and apoptosis: high-dose toxicity in normal cells.
Nanomedicine (Lond)
; 18(11): 875-887, 2023 05.
Article
en En
| MEDLINE
| ID: mdl-37470184
ABSTRACT
Aim:
The cytotoxic effects of graphene oxide nanoparticles (GONPs) using MTT assays, observance of apoptotic markers, and oxidative stress were outlined. Materials &methods:
Rat embryonic fibroblasts (REFs) and human epithelial breast cells (HBLs) were used at 250, 500 and 750 µg/ml concentrations.Results:
Significant cytotoxic and apoptotic effects were observed. Analyses of CYP2E1 and malondialdehyde concentrations in REF and HBL-100 cell lines after exposing to GONPs confirmed the nanomaterials toxicity. However, the glutathione levels in REF and HBL-100 cell lines showed a substantial reduction compared with the control. The cytochrome CYP2E1, glutathione, malondialdehyde and caspase-3 alterations provided a plausible interlinked relationship.Conclusion:
The study confirmed the GONPs cytotoxic effects on REF and HBL-100 cell lines. The outcome suggested caution in wide-spread applications of GONPs, which could have implications for occupational health also.Palabras clave
Texto completo:
1
Colección:
01-internacional
Asunto principal:
Citocromo P-450 CYP2E1
/
Antineoplásicos
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Nanomedicine (Lond)
Año:
2023
Tipo del documento:
Article
País de afiliación:
Irak