Representative measles virus infection requires appropriate airway epithelia culture conditions.
J Virol
; 97(10): e0105123, 2023 10 31.
Article
en En
| MEDLINE
| ID: mdl-37732787
ABSTRACT
IMPORTANCE For many years, measles virus (MeV) was assumed to first enter the host via the apical surface of airway epithelial cells and subsequently spread systemically. We and others reported that MeV has an overwhelming preference for entry at the basolateral surface of airway epithelial cells, which led to a fundamental new understanding of how MeV enters a human host. This unexpected observation using well-differentiated primary cultures of airway epithelia from human donors contradicted previous studies using immortalized cultured cells. Here, we show that appropriate differentiation and cell morphology of primary human airway epithelial cells are critical to recapitulate MeV infection patterns and pathogenesis of the in vivo airways. By simply culturing primary cells in media containing serum or passaging primary cultures, erroneous results quickly emerge. These results have broad implications for data interpretation related to respiratory virus infection, spread, and release from human airway epithelial cells.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Asunto principal:
Sistema Respiratorio
/
Células Cultivadas
/
Células Epiteliales
/
Sarampión
/
Virus del Sarampión
Límite:
Humans
Idioma:
En
Revista:
J Virol
Año:
2023
Tipo del documento:
Article
País de afiliación:
Estados Unidos