Your browser doesn't support javascript.
loading
Radiation induces acute and subacute vascular regression in a three-dimensional microvasculature model.
Choi, Dong-Hee; Oh, Dongwoo; Na, Kyuhwan; Kim, Hyunho; Choi, Dongjin; Jung, Yong Hun; Ahn, Jinchul; Kim, Jaehoon; Kim, Chun-Ho; Chung, Seok.
Afiliación
  • Choi DH; School of Mechanical Engineering, Korea University, Seoul, Republic of Korea.
  • Oh D; R&D Research Center, Next&Bio Inc, Seoul, Republic of Korea.
  • Na K; Korea University-Korea institute of Science and Technology (KU-KIST) Graduate School of Converging Science and Technology, Korea University, Seoul, Republic of Korea.
  • Kim H; School of Mechanical Engineering, Korea University, Seoul, Republic of Korea.
  • Choi D; R&D Research Center, Next&Bio Inc, Seoul, Republic of Korea.
  • Jung YH; School of Mechanical Engineering, Korea University, Seoul, Republic of Korea.
  • Ahn J; Center for Systems Biology, Massachusetts General Hospital, Boston, MA, United States.
  • Kim J; Laboratory of Tissue Engineering, Korea Institute of Radiological and Medical Sciences, Seoul, Republic of Korea.
  • Kim CH; School of Mechanical Engineering, Korea University, Seoul, Republic of Korea.
  • Chung S; R&D Research Center, Next&Bio Inc, Seoul, Republic of Korea.
Front Oncol ; 13: 1252014, 2023.
Article en En | MEDLINE | ID: mdl-37909014
ABSTRACT
Radiation treatment is one of the most frequently used therapies in patients with cancer, employed in approximately half of all patients. However, the use of radiation therapy is limited by acute or chronic adverse effects and the failure to consider the tumor microenvironment. Blood vessels substantially contribute to radiation responses in both normal and tumor tissues. The present study employed a three-dimensional (3D) microvasculature-on-a-chip that mimics physiological blood vessels to determine the effect of radiation on blood vessels. This model represents radiation-induced pathophysiological effects on blood vessels in terms of cellular damage and structural and functional changes. DNA double-strand breaks (DSBs), apoptosis, and cell viability indicate cellular damage. Radiation-induced damage leads to a reduction in vascular structures, such as vascular area, branch length, branch number, junction number, and branch diameter; this phenomenon occurs in the mature vascular network and during neovascularization. Additionally, vasculature regression was demonstrated by staining the basement membrane and microfilaments. Radiation exposure could increase the blockage and permeability of the vascular network, indicating that radiation alters the function of blood vessels. Radiation suppressed blood vessel recovery and induced a loss of angiogenic ability, resulting in a network of irradiated vessels that failed to recover, deteriorating gradually. These findings demonstrate that this model is valuable for assessing radiation-induced vascular dysfunction and acute and chronic effects and can potentially improve radiotherapy efficiency.
Palabras clave

Texto completo: 1 Colección: 01-internacional Idioma: En Revista: Front Oncol Año: 2023 Tipo del documento: Article

Texto completo: 1 Colección: 01-internacional Idioma: En Revista: Front Oncol Año: 2023 Tipo del documento: Article