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Silver nanoparticles stimulate 5-Fluorouracil-induced colorectal cancer cells to kill through the upregulation TRPV1-mediated calcium signaling pathways.
Kaya, Müge Mavioglu.
Afiliación
  • Kaya MM; Department of Molecular Biology and Genetics, Faculty of Science, Kafkas University, Kars, Türkiye.
Cell Biol Int ; 48(5): 712-725, 2024 May.
Article en En | MEDLINE | ID: mdl-38499507
ABSTRACT
The involvement of the TRP vanilloid 1 (TRPV1) cation channel on the 5-Fluorouracil (5-FU)-caused Ca2+ signals through the activation of the apoptotic signaling pathway and stimulating the mitochondrial Ca2+ and Zn2+ accumulation-induced reactive oxygen species (ROS) productions in several cancer cells, except the colorectal cancer (HT-29) cell line, was recently reported. I aimed to investigate the action of silver nanoparticles (SiNPs) and 5-FU incubations through the activation of TRPV1 on ROS, apoptosis, and cell death in the HT-29 cell line. The cells were divided into four groups control, SiNP (100 µM for 48 h), 5-FU (25 µM for 24 h), and 5-FU + SiNP. SiNP treatment through TRPV1 activation (via capsaicin) stimulated the oxidant and apoptotic actions of 5-FU in the cells, whereas they were diminished in the cells by the TRPV1 antagonist (capsazepine) treatment. The apoptotic and cell death actions of 5-FU were determined by increasing the propidium iodide/Hoechst rate, caspase-3, -8, and -9 activations, mitochondrial membrane depolarization, lipid peroxidation, and ROS, but decreasing the glutathione and glutathione peroxidase. The increase of cytosolic free Ca2+ and Zn2+ into mitochondria via the stimulation of TRPV1 current density increased oxidant and apoptotic properties of 5-FU in the cells. For the therapy of HT-29 tumor cells, I found that the combination of SiNPs and 5-FU was synergistic via TRPV1 activation.
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Texto completo: 1 Colección: 01-internacional Asunto principal: Neoplasias Colorrectales / Nanopartículas del Metal / Antineoplásicos Límite: Humans Idioma: En Revista: Cell Biol Int Año: 2024 Tipo del documento: Article

Texto completo: 1 Colección: 01-internacional Asunto principal: Neoplasias Colorrectales / Nanopartículas del Metal / Antineoplásicos Límite: Humans Idioma: En Revista: Cell Biol Int Año: 2024 Tipo del documento: Article