Generation and characterization of the iPS cell line (SYSUSHi001-A) derived from the peripheral blood mononuclear cells (PBMCs) of a 33-year-old patient with acute myeloid leukemia (AML).
Stem Cell Res
; 77: 103360, 2024 Jun.
Article
en En
| MEDLINE
| ID: mdl-38608356
ABSTRACT
We successfully developed an induced pluripotent stem cell (iPSC) line, SYSUSHi001-A, from the peripheral blood mononuclear cells (PBMC) of a patient with Acute Myeloid Leukemia, harboring two genetic mutations (XPO1 c.591-4_591-3dupTT; PALB2 c.3296C > T; p.T1099M). This iPSC line was facilitated through the use of episomal plasmids encoding OCT4, SOX2, KLF4, L-MYC, and human miR-302. The SYSUSHi001-A iPSC line exhibited characteristic embryonic stem cell-like morphology, maintained the XPO1 and PALB2 mutations, expressed key pluripotency markers, preserved a normal karyotype (46, XY), and demonstrated the ability to differentiate into cells from all three germ layers in vitro.
Texto completo:
1
Colección:
01-internacional
Asunto principal:
Leucocitos Mononucleares
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Leucemia Mieloide Aguda
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Células Madre Pluripotentes Inducidas
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Factor 4 Similar a Kruppel
Límite:
Adult
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Humans
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Male
Idioma:
En
Revista:
Stem Cell Res
/
Stem cell research (Online)
Año:
2024
Tipo del documento:
Article
País de afiliación:
China