A Radical-Generating Probe to Release Free Fluorophores and Identify Artemisinin-Sensitive Cancer Cells.
ACS Sens
; 9(5): 2310-2316, 2024 05 24.
Article
en En
| MEDLINE
| ID: mdl-38651676
ABSTRACT
The smart light-up probes have been extensively developed to image various enzymes and other bioactive molecules. Upon activation, these probes result in light-up fluorophores that exist in a protein-bound or a free form. The difference between these two forms has not yet been reported. Here, we present a pair of smart light-up probes that generate a protein-bound fluorophore and a free fluorophore upon activation by heme. Probe 8 generated a radical-attached fluorophore that predominantly existed in the free form, while probe 10 generated an α,ß-unsaturated ketone-attached fluorophore that showed extensive labeling of proteins. In live-cell imaging, probe 8 showed greater fluorescence intensity than probe 10 when low concentrations (0.1-5 µM) of the probes were used, but probe 8 was less fluorescent than probe 10 when the concentrations of the probes were high (10 µM). Finally, probe 8 was used to reflect the activation level of the endoperoxide bond in cancer cells and to effectively distinguish ART-sensitive cancer cells from ART-insensitive ones.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Asunto principal:
Artemisininas
/
Colorantes Fluorescentes
Límite:
Humans
Idioma:
En
Revista:
ACS Sens
Año:
2024
Tipo del documento:
Article
País de afiliación:
China