Ssc-miR-7139-3p suppresses foot-and-mouth disease virus replication by promoting degradation of 3Cpro through targeting apoptosis-negative regulatory gene Bcl-2.
Virology
; 595: 110070, 2024 Jul.
Article
en En
| MEDLINE
| ID: mdl-38657363
ABSTRACT
Foot-and-mouth disease is a highly contagious and infectious disease affecting cloven-hoofed animals. Disease control is complicated by its highly contagious nature and antigenic diversity. Host microRNAs (miRNAs) are post-transcriptional regulators that either promote or repress viral replications in virus infection. In the present study, we found that ssc-miR-7139-3p (Sus scrofa miR-7139-3p) was significantly up-regulated in host cells during foot-and-mouth disease virus (FMDV) infection. Overexpression of miR-7139-3p attenuated FMDV replication, whereas inhibition promoted FMDV replication. In addition, the survival rate of FMDV infected suckling mice was increased through injection of miR-7139-3p agomiR. Further studies revealed that miR-7139-3p targets Bcl-2 to initiate the apoptotic pathway and caspase-3 cleaved 3Cpro behind the 174th aspartic acid (D174), which eventually promotes the degradation of 3Cpro. Overall, our findings demonstrate that miR-7139-3p suppresses FMDV replication by promoting degradation of 3Cpro through targeting the apoptosis-negative regulatory gene Bcl-2.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Asunto principal:
Replicación Viral
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Apoptosis
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Proteínas Proto-Oncogénicas c-bcl-2
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Virus de la Fiebre Aftosa
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MicroARNs
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Fiebre Aftosa
Límite:
Animals
Idioma:
En
Revista:
Virology
Año:
2024
Tipo del documento:
Article
País de afiliación:
China