A near-infrared fluorescent probe for the detection of Cu2+ in Chinese herbal medicine and imaging in living cells.
Spectrochim Acta A Mol Biomol Spectrosc
; 317: 124407, 2024 Sep 05.
Article
en En
| MEDLINE
| ID: mdl-38723466
ABSTRACT
Copper is one of the common among the heavy metal pollution in Chinese herbal medicine (CHM). So, it is essential to develop rapid and accurate testing method to quantify the Cu2+ content in CHM. Herein, we prepared a coordination-based near-infrared fluorescent probe (NRh6G-FA) by introducing a hemicyanine dye in rhodamine 6G scaffold. NRh6G-FA had a high sensitivity, anti-interference performance, fast response (within 60 s), visualization (from light yellow to green) for Cu2+ and excellent sensing performance for the detection of Cu2+ at low concentrations (LOD = 0.225 µM). The most likely mechanism was verified on the basis of Job's plot, ESI-HRMS and DFT calculations. NRh6G-FA could be successfully applied for the detection and "naked eye" recognition of Cu2+ in CHM samples. Moreover, NRh6G-FA was used to visualize Cu2+ in living MCF-7 cells by confocal fluorescence imaging.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Asunto principal:
Medicamentos Herbarios Chinos
/
Cobre
/
Colorantes Fluorescentes
Límite:
Humans
Idioma:
En
Revista:
Spectrochim Acta A Mol Biomol Spectrosc
Asunto de la revista:
BIOLOGIA MOLECULAR
Año:
2024
Tipo del documento:
Article