Apoplast-Extraction Based Method to Improve the Purity of Plant Produced Recombinant Protein.
J Vis Exp
; (209)2024 Jul 05.
Article
en En
| MEDLINE
| ID: mdl-39037257
ABSTRACT
Plants are a newly developing eukaryotic expression system being explored to produce therapeutic proteins. Purification of recombinant proteins from plants is one of the most critical steps in the production process. Typically, proteins were purified from total soluble proteins (TSP), and the presence of miscellaneous intracellular proteins and cytochromes poses challenges for subsequent protein purification steps. Moreover, most therapeutic proteins like antigens and antibodies are secreted to obtain proper glycosylation, and the presence of incompletely modified proteins leads to inconsistent antigen or antibody structures. This work introduces a more effective method to obtain highly purified recombinant proteins from the plant apoplastic space. The recombinant Green fluorescent protein (GFP) is engineered to be secreted into the apoplast of Nicotiana benthamiana and is then extracted using an infiltration-centrifugation method. The GFP-His from the extracted apoplast is then purified by nickel affinity chromatography. In contrast to the traditional methods from TSP, purification from the apoplast produces highly purified recombinant proteins. This represents an important technological improvement for plant production systems.
Texto completo:
1
Colección:
01-internacional
Asunto principal:
Nicotiana
/
Cromatografía de Afinidad
/
Proteínas Fluorescentes Verdes
Idioma:
En
Revista:
J Vis Exp
Año:
2024
Tipo del documento:
Article