[Inhibition Effect of Resveratrol on Spontaneous Senescence of Human Bone Marrow Derived Mesenchymal Stem Cells].
Zhongguo Shi Yan Xue Ye Xue Za Zhi
; 32(4): 1224-1229, 2024 Aug.
Article
en Zh
| MEDLINE
| ID: mdl-39192424
ABSTRACT
OBJECTIVE:
To explore whether Resveratrol (RSV) can inhibit the spontaneous senescence of human bone marrow-derived mesenchymal stem cells (MSC).METHODS:
MSC were serially cultured to passage 13 and passage 15 to establish model groups exhibiting spontaneous senescence, respectively. MSC at passage 13 and passage 15 were treated with 5 nmol/L RSV for 48 h to establish the RSV-treated groups. SA-ß-Gal staining was used to detect cell senescence. MTT assay was used to detect cell proliferation. RT-PCR method was used to detect senescenceîassociated telomerase activity. Western blot was used to detect the senescence-associated protein level of the phosphorylated-mTOR.RESULTS:
SA-ß-Gal staining showed that the senescent cells of MSC in RSV-treated group was significantly less than those in the model group (RSV group compared with model group at passage 13, P < 0.05; RSV group compared with model group at passage 15, P < 0.01). The cell proliferation ability of MSC in RSV-treated group was significantly higher than those in model group, at 72 h in passage 13, there was significant difference between RSV-treated group and model group (P < 0.05). RT-PCR results showed that the hTERT mRNA expression of MSC in RSV-treated group was higher than that in model group, which was significantly different between RSV-treated group and model group at passage 13 (P < 0.05). Western blot results showed that the phosphorylated (Ser2448)-mTOR level of MSC in RSV-treated group was lower than that in model group, which was significantly different between RSV-treated group and model group at passage 13 (P < 0.05).CONCLUSION:
RSV can inhibit the spontaneous senescence of human MSC by mediating mTOR activity.Palabras clave
Texto completo:
1
Colección:
01-internacional
Asunto principal:
Células de la Médula Ósea
/
Senescencia Celular
/
Telomerasa
/
Proliferación Celular
/
Serina-Treonina Quinasas TOR
/
Células Madre Mesenquimatosas
/
Resveratrol
Límite:
Humans
Idioma:
Zh
Revista:
Zhongguo Shi Yan Xue Ye Xue Za Zhi
Asunto de la revista:
HEMATOLOGIA
Año:
2024
Tipo del documento:
Article
País de afiliación:
China